Partial separation of gonadotropin-inhibiting substance in human urine.

The chromatographic separation of gonadotropin-inhibiting substance in the extract obtained from adult male urine according to the method of Johnsen was attempted on a Sephadex G-100 column. The ovulation assay was employed to detect the inhibitor in the fractions using female mice, 19-20 days old, which received PMS and HCG to obtain a minimum constant ovulation. In the chromatography of the extract, the inhibitor mainly emerged in the early fractions or in the excluded volume , while HPG appeared in the later fractions which induced super-ovulation when injected with the standard dose of HCG. A slight inhibition on the ovulation was observed in the far later fractions.